Ion Exchange Chromatography

In water analysis, quality control, and protein purification, ion exchange chromatography is usually used. Ion exchange chromatography is defined as the process of separating polar molecules and ions based on charge. This process may be used for any charged molecule like amino acids, large proteins, and small nucleotides.

There are different methods used in chromatography, and the term ‘ion exchange chromatography’ is applicable to the separation of analytes’ components for analytical applications.

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High Performance Liquid Chromatography

Even when you first heard of High Performance Liquid Chromatography, the name explains that it is a sophisticated operation of column chromatography. Chromatography or in lay person’s term, refers to a separation technique in chemical analysis. If you recall your high school chemistry, column chromatography is a method used to separate a pure compound from a mixture of chemical compounds using gravity. Similarly works in High Performance Liquid Chromatography procedure, except the mixture of compounds is forced through the system under high pressures up to 5000 psi, thus HPLC at times are commonly mistaken as High Pressure Liquid Chromatography!

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Ion Exchange Chromatography: How it works?

Belongs to the family of chromatography, Ion Exchange Chromatography or sometimes refers to only Ion Chromatography (IC) is also a separation technique, technically a column chromatography that plays by the rule of the ‘opposite attracts’. Slightly difference compares to HPLC or Gas Chromatography, it exploits the interactions of ions and polar molecules, Ion Exchange Chromatography can be used to separate almost all types of molecules as long as these analytes are charged. Widely applied in the fields of protein purification, water analysis, quality control and research laboratories of large proteins to small nucleotides and amino acids.

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